Introduction Preparing for the Unit Introduction to the data analysis program The concept of gating Using Boolean logic  Sequential gating Back gating Colour gating Quadrant regions CD45 gating Statistical Analysis Self Assessment Introductory Contents Further reading Units 	Introduction  The flow cytometer 	Data analysis 	Immunofluorescence 	Some clinical applications  DNA and the cell cycle Other applications Cell proliferation Cell death Assignment 1 Assignment 2

FITC

PE

PC5

PC7


0

CD4

CD45RO

CD45RA

FITC control

CD8

0

CD45EO

CD45RA

PE control

CD8

CD4

0

CD45RA

PC5 control

CD8

CD4

CD45RO

0

PC7 control

continued