Introductory Contents Further reading Units 	Introduction  The flow cytometer 	Data analysis 	Immunofluorescence 	Some clinical applications  DNA and the cell cycle Other applications Cell proliferation Cell death Assignment 1 Assignment 2 continued

Compound

Abbreviation

  Emission wavelength in nm

Colour

Excitation at 488 nm  



fluorescein

FITC

520

green

Alexa 488


515

green

phycoerythrin

PE

565

yellow

phycoerythrin-Texas Red

ECD

620

red

phycoerythrin-cyanine 5

PC5

665

Deep red

Peridinin chlorophyll

PERCP

677

deep red

phycoerythrin-cyanine 5.5

PC5.5

696

 deep red

phycoerythrin-cyanine 7

PC7

756

far red

Excitation at 630 nm



allophycocyanin

APC

660

deep red

allophycocyanin-cyanine 5.5

APC-Cy5.5

696

deep red

allophycocyanin-cyanine 7

APC-Cy7


far red

Excitation at 405




Cascade Blue


419

violet

Pacific Blue


456

blue

Cascade Yellow


549