What is flow cytometry?

The flow system

Light scatter

Fluorescence measurement

Multiparametric measurement

Advantages of flow cytometry

Disadvantages of flow cytometry

Sample preparation

Applications

Data display

Safety

Further reading

Self Assessment

Introductory Contents Further reading Units 	Introduction  The flow cytometer 	Data analysis 	Immunofluorescence 	Some clinical applications  DNA and the cell cycle Other applications Cell proliferation Cell death Assignment 1 Assignment 2

If, for any reason, the laser beam is exposed, special precautions must be taken. The laser beams will burn the retina of the eye and cause permanent damage. They should normally be fully enclosed unless they are being aligned, normally by a trained engineer.

During alignment, any uninvolved personnel should be excluded from the room. No shiny metal object (which can reflect a laser beam into someone's eye) should be brought near to a laser beam; rings and bracelets should be removed or securely covered.

Safety goggles are available and should be worn whenever practicable when working with exposed beams. Local rules for the operation of lasers should be discussed and agreed with your laser safety officer.

Further reading

Givan, A.L. (2001) Flow Cytometry. First Principles. 2nd edition. Wiley-Liss, New York.

Shapiro, H.M. (2002) Practical Flow Cytometry. 4th edition. Wiley-Liss, Inc., New York.


END OF UNIT 1

Self Assessment