If, for any reason, the laser beam is exposed, special precautions must be taken. The laser beams will burn the retina of the eye and cause permanent damage. They should normally be fully enclosed unless they are being aligned, normally by a trained engineer.
During alignment, any uninvolved personnel should be excluded from the room. No shiny metal object (which can reflect a laser beam into someone's eye) should be brought near to a laser beam; rings and bracelets should be removed or securely covered.
Safety goggles are available and should be worn whenever practicable when working with exposed beams. Local rules for the operation of lasers should be discussed and agreed with your laser safety officer.
Givan, A.L. (2001) Flow Cytometry. First Principles. 2nd edition. Wiley-
Shapiro, H.M. (2002) Practical Flow Cytometry. 4th edition. Wiley-
END OF UNIT 1